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  • Alex Kentsis

  • May 29, 2015

Fabrication of Capillary Columns with Integrated Frits for Mass Spectrometry

Miniaturization of columns for liquid chromatography has greatly enhanced its sensitivity and resolution. Here, we provide a detailed protocol that describes the fabrication of fused silica capillaries with frits and their packing with chromatography media for high-resolution, high-capacity separations suitable for nanoscale liquid-chromatography mass spectrometry. The protocol can be downloaded as PDF, and is available from Nature Protocol Exchange.