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  • Alex Kentsis

  • Jul 24, 2015

Native proteome extraction using poloxamer-based adaptive focused sonication

Recent developments in quantitative high-resolution mass spectrometry have led to significant improvements in the sensitivity and specificity of biochemical analyses of cellular reactions, protein-protein interactions, and small molecule drug discovery. These approaches depend on cellular proteome extraction that preserves native protein activities. This protocol describes a method that combines adaptive focused acoustic sonication and a binary poloxamer-based lysis buffer to maximize extraction of cellular proteins while maintaining native protein activity, as published in Dhabaria et al. A high-efficiency cellular extraction system for biological proteomics. The protocol can be downloaded as PDF, and is available from Nature Protocol Exchange.