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Category archives for Protocol

Oct 04, 2018

Reliable commercial antibodies for PGBD5

A few years ago, we published a paper reporting PGBD5 as an oncogenic mutator in human cancer. This included measuring its expression in human tissues and mapping its association with…

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Jul 25, 2018

Engineering scarless chromosomal rearrangements using homology-mediated recombination and selection

Genetically engineered mouse models of human cancers have proven to be essential for the elucidation of basic molecular mechanisms of cancer pathogenesis and their therapeutic targeting. However, for many genetic…

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Jul 24, 2015

Native proteome extraction using poloxamer-based adaptive focused sonication

Recent developments in quantitative high-resolution mass spectrometry have led to significant improvements in the sensitivity and specificity of biochemical analyses of cellular reactions, protein-protein interactions, and small molecule drug discovery.…

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Jul 21, 2015

Transposon mapping using flanking sequence exponential anchored (FLEA) PCR

This protocol describes a modified version of flanking sequence exponential anchored (FLEA) PCR. The original FLEA-PCR method was developed to amplify integration sites of retroviruses or retro-transposons. Such transposons contain…

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May 29, 2015

Fabrication of Capillary Columns with Integrated Frits for Mass Spectrometry

Miniaturization of columns for liquid chromatography has greatly enhanced its sensitivity and resolution. Here, we provide a detailed protocol that describes the fabrication of fused silica capillaries with frits and their packing with…

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