Recent developments in quantitative high-resolution mass spectrometry have led to significant improvements in the sensitivity and specificity of biochemical analyses of cellular reactions, protein-protein interactions, and small molecule drug discovery.…
Read More
This protocol describes a modified version of flanking sequence exponential anchored (FLEA) PCR. The original FLEA-PCR method was developed to amplify integration sites of retroviruses or retro-transposons. Such transposons contain…
Read MoreMiniaturization of columns for liquid chromatography has greatly enhanced its sensitivity and resolution. Here, we provide a detailed protocol that describes the fabrication of fused silica capillaries with frits and their packing with…
Read MoreIonization efficiency and consequently the sensitivity of mass spectrometric analyses strongly depend on the geometry of nanoelectrospray emitter used, particularly on the terminal inner diameter (ID) of the tip. Here,…
Read MoreIF EPIGENETIC HULK READ ONE MORE PAPER ON NAIVE PLURIPOTENCY HE GET REALLY GREEN! NOBODY CARE ABOUT CELL CULTURE ARTIFACT! STOP WASTE TIME!
SMARCB1 is required for widespread BAF complex–mediated activation of enhancers and bivalent promoters https://t.co/tCPxPNCqKv by @CKadoch
John Abrams et al on p53 in the game of transposons, defining transposoapathies with video https://t.co/UeKejBDQth https://t.co/396fPYZPhc
Getting tired reading various methods for prediction of MHC peptides. Folks should simply measure them directly using mass spectrometry.
