Recent developments in quantitative high-resolution mass spectrometry have led to significant improvements in the sensitivity and specificity of biochemical analyses of cellular reactions, protein-protein interactions, and small molecule drug discovery.…
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This protocol describes a modified version of flanking sequence exponential anchored (FLEA) PCR. The original FLEA-PCR method was developed to amplify integration sites of retroviruses or retro-transposons. Such transposons contain…
Read MoreMiniaturization of columns for liquid chromatography has greatly enhanced its sensitivity and resolution. Here, we provide a detailed protocol that describes the fabrication of fused silica capillaries with frits and their packing with…
Read MoreIonization efficiency and consequently the sensitivity of mass spectrometric analyses strongly depend on the geometry of nanoelectrospray emitter used, particularly on the terminal inner diameter (ID) of the tip. Here,…
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