Sample preparation remains essential for high-quality mass spectrometry proteomics. Here, we compared three commonly used methods for protein extraction of frozen mammalian tissues: 1) extraction using SDS buffer followed by…
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While computational genomics methods have been greatly advanced by open development in distributed computing environments, computational tools for mass spectrometry proteomics remain tethered to proprietary and often outdated operating systems.…
Read MoreA few years ago, we published a paper reporting PGBD5 as an oncogenic mutator in human cancer. This included measuring its expression in human tissues and mapping its association with…
Read MoreGenetically engineered mouse models of human cancers have proven to be essential for the elucidation of basic molecular mechanisms of cancer pathogenesis and their therapeutic targeting. However, for many genetic…
Read MoreRecent developments in quantitative high-resolution mass spectrometry have led to significant improvements in the sensitivity and specificity of biochemical analyses of cellular reactions, protein-protein interactions, and small molecule drug discovery.…
Read MoreSometimes it’s important to publish your results when the data doesn’t turn out how you hoped. Here’s our paper evaluating the quantitative consequences of using a protein carrier in two low-input applications: pMHC and pTyr…
https://doi.org/10.1016/j.mcpro.2021.100104
A first-in-class polymerase theta inhibitor selectively targets homologous-recombination-deficient tumors
https://www.nature.com/articles/s43018-021-00203-x
@NatureCancer
How do DNA-binding proteins engage specific sites “hidden” in chromatin? Recent cryoEM studies shed light on mechanisms @KurumizakaLab @CramerLab TaipaleLab, @SchubelerLab @LabThoma
https://authors.elsevier.com/a/1dGBbL7PXe%7Eh%7E
Proteomics folks, we are trying to look for mass spectral evidence of branched peptides, where fragment spectra may content a large lysine adduct and many will be chimeric from co-fragmentation of branches. 1/n
