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Category archives for Protocol

Jul 24, 2015

Native proteome extraction using poloxamer-based adaptive focused sonication

Recent developments in quantitative high-resolution mass spectrometry have led to significant improvements in the sensitivity and specificity of biochemical analyses of cellular reactions, protein-protein interactions, and small molecule drug discovery.…

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Jul 21, 2015

Transposon mapping using flanking sequence exponential anchored (FLEA) PCR

This protocol describes a modified version of flanking sequence exponential anchored (FLEA) PCR. The original FLEA-PCR method was developed to amplify integration sites of retroviruses or retro-transposons. Such transposons contain…

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May 29, 2015

Fabrication of Capillary Columns with Integrated Frits for Mass Spectrometry

Miniaturization of columns for liquid chromatography has greatly enhanced its sensitivityΒ and resolution. Here, we provide a detailedΒ protocol that describes the fabrication of fused silica capillaries with fritsΒ and their packing with…

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May 29, 2015

Fabrication of Nanoelectrospray Emitters for LC-MS

Ionization efficiency and consequently the sensitivity of mass spectrometric analyses strongly depend on the geometry of nanoelectrospray emitter used, particularly on the terminal inner diameter (ID) of the tip. Here,…

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