• Alex Kentsis

  • Jul 21, 2015

Transposon mapping using flanking sequence exponential anchored (FLEA) PCR

This protocol describes a modified version of flanking sequence exponential anchored (FLEA) PCR. The original FLEA-PCR method was developed to amplify integration sites of retroviruses or retro-transposons. Such transposons contain two long terminal repeats (LTR), which have the same orientation. In contrast, DNA transposons have inverted terminal repeats (ITR), which are inverted in their orientation. Here, we adapt FLEA-PCR for mapping genomic DNA transposition, suitable for the analysis of DNA transposition reporters and genomes engineered through the use of DNA transposition. The protocol can be downloaded as PDF, and is available from Nature Protocol Exchange.